Difference between revisions of "Part:BBa F2620"
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| − | ''A work in progress... | + | ''A work in progress...'' |
<div style="padding: 40px; width: 720px; border: 1px solid #000000;"> | <div style="padding: 40px; width: 720px; border: 1px solid #000000;"> | ||
| + | {| | ||
| + | |width="250px"|<span style="font-family: Arial Black; font-size: 18pt">BBa_F2620</span><br> | ||
| − | <div style="padding: 00px; width: 720px; border: | + | |
| + | <span style="font-family: Arial; font-size: 12pt">3OC<sub>6</sub>HSL -> PoPS Receiver</span><br> | ||
| + | |[[Image:F2620.jpg|200px]] | ||
| + | |} | ||
| + | <span style="align: left> | ||
| + | Authors: Barry Canton [bcanton@mit.edu], Anna Labno [labnoa@mit.edu] Last Update: Sept. 15th, 2006 | ||
| + | </span> | ||
| + | ---- | ||
| + | |||
| + | <div style="padding: 00px; width: 720px; border: 0px solid #000000;"> | ||
'''Description'''<br> | '''Description'''<br> | ||
A transcription factor [LuxR] that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC6HSL]] is controlled by an operator [TetR]. ''Device input'' is [[3OC6HSL|3OC6HSL]]. ''Device output'' is [[PoPS]] produced at a LuxR-regulated operator. | A transcription factor [LuxR] that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC6HSL]] is controlled by an operator [TetR]. ''Device input'' is [[3OC6HSL|3OC6HSL]]. ''Device output'' is [[PoPS]] produced at a LuxR-regulated operator. | ||
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Full [[PoPS]] output at high [[3OC6HSL]] levels and high plasmid copy [e.g., [[Part:pSB1A2]]] results in a reduced cell growth rate (see Load section). If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function. | Full [[PoPS]] output at high [[3OC6HSL]] levels and high plasmid copy [e.g., [[Part:pSB1A2]]] results in a reduced cell growth rate (see Load section). If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function. | ||
</div> | </div> | ||
| − | {|align="center" style="padding: 0px" | + | {|align="center" style="padding: 0px" cellspacing="0" cellpadding="0" |
|width='360px'|'''Characteristics''' | |width='360px'|'''Characteristics''' | ||
| − | {|align="left | + | {|align="left" |
|width='85px'|[[Input Swing|''Input Swing:'']] | |width='85px'|[[Input Swing|''Input Swing:'']] | ||
|width='265px'|## nM 3OC<sub>6</sub>HSL, exogenous | |width='265px'|## nM 3OC<sub>6</sub>HSL, exogenous | ||
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|} | |} | ||
|width='360px' align="right" valign="top"|'''Key Subparts'''<br> | |width='360px' align="right" valign="top"|'''Key Subparts'''<br> | ||
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|width='85px'|''<partinfo>R0040</partinfo>:'' | |width='85px'|''<partinfo>R0040</partinfo>:'' | ||
|TetR-regulated operator | |TetR-regulated operator | ||
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|} | |} | ||
| − | {|align="center" | + | |
| + | {|align="center" cellspacing="0" cellpadding="0" | ||
|width='375px' align="left"|'''Transfer Function'''<br>[[Image:BC-TransferCurve-3.png|300px]] | |width='375px' align="left"|'''Transfer Function'''<br>[[Image:BC-TransferCurve-3.png|300px]] | ||
|width='375px' align="right"|'''Specificity'''<br>[[Image:BC-Specificity-1.png|300px]] | |width='375px' align="right"|'''Specificity'''<br>[[Image:BC-Specificity-1.png|300px]] | ||
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|} | |} | ||
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| − | {|align="center" | + | {|align="center" cellspacing="0" cellpadding="0" |
|width='360px' valign="top"|'''Demand''' | |width='360px' valign="top"|'''Demand''' | ||
| − | {|align="left | + | {|align="left" |
| − | |width=' | + | |width='125px' valign="top"|[[Transcription Demand|''Transcription Demand:'']] |
| − | |width=' | + | |width='235px'valign="top"|## NTP per second<br>## polymerases sequestered |
|- | |- | ||
| − | |[[Translation Demand|''Translation Demand:'']] | + | |valign="top"| [[Translation Demand|''Translation Demand:'']] |
| − | |# charged tRNA per second | + | |valign="top"| # charged tRNA per second<br># ribosomes sequestered |
|} | |} | ||
|width='360px' align="right" valign="top"|'''Stability'''<br> | |width='360px' align="right" valign="top"|'''Stability'''<br> | ||
| − | {|align="right | + | {|align="right" |
| − | |width=' | + | |width='140px' valign="top"|[[Genetic Stability|''Genetic Stability:'']] |
| − | |##/## replication events (Lo Input/High Input) | + | |valign="top"| ##/## replication events (Lo Input/High Input) |
|- | |- | ||
| − | |[[Performance Stability|''Performance Stability:'']] | + | |valign="top"|[[Performance Stability|''Performance Stability:'']] |
| − | |##/## replication events (Lo Input/High Input) | + | |valign="top"|##/## replication events (Lo Input/High Input) |
|} | |} | ||
|} | |} | ||
</div> | </div> | ||
| − | <div style="padding: 00px; width: 720px | + | <div style="padding: 00px; width: 720px"> |
'''Compatibility'''<br> | '''Compatibility'''<br> | ||
Device has been shown to work in ''<partinfo>BBa_V1002</partinfo>'', ''MG1655'' and ''<partinfo>BBa_V1001</partinfo>''<br> | Device has been shown to work in ''<partinfo>BBa_V1002</partinfo>'', ''MG1655'' and ''<partinfo>BBa_V1001</partinfo>''<br> | ||
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<partinfo>BBa_F2620 short</partinfo> | <partinfo>BBa_F2620 short</partinfo> | ||
| − | + | <partinfo>BBa_F2620 SpecifiedComponents</partinfo> | |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_F2620 SequenceAndFeatures</partinfo> | <partinfo>BBa_F2620 SequenceAndFeatures</partinfo> | ||
Revision as of 21:26, 15 September 2006
A work in progress...
| BBa_F2620
|
|
Authors: Barry Canton [bcanton@mit.edu], Anna Labno [labnoa@mit.edu] Last Update: Sept. 15th, 2006
Description
A transcription factor [LuxR] that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by an operator [TetR]. Device input is 3OC6HSL. Device output is PoPS produced at a LuxR-regulated operator.
Usage
Full PoPS output at high 3OC6HSL levels and high plasmid copy [e.g., Part:pSB1A2] results in a reduced cell growth rate (see Load section). If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.
Characteristics
|
Key Subparts
|
Transfer Function
|
Specificity
|
Latency
|
Stability
|
Demand
|
Stability
|
Compatibility
Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Device has been shown to work on pSB3K3 and pSB3K3
Device has been shown to work with BBa_E0430,BBa_E0434,BBa_E0240
Crosstalk with devices using 3OC6HSL or add in the other molecule names here.
Crosstalk with systems containing TetR (BBa_C0040)
3OC6HSL -> PoPS Receiver
| p(tetR) R0040 | B0034 | luxr C0062 | B0015 | lux pR R0062 | |
 |  |  |  | |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1004
Device Characteristics
| control | R0040 |
| device_type | Receiver |
| family | -NA- |
| latency | Seconds |
| proteins | LuxR, TetR |
| signalling_molecule | 3OC6HSL |
| switch_point | 2nM |
Get the device [http://openwetware.org/wiki/Image:F2620.de2-ania.ppt#filelinks datasheet]
Transfer Function
Latency
Specificity
[http://openwetware.org/images/9/93/Transfer.jpg Specificity preliminary data]
Stability
[http://openwetware.org/images/b/b9/StabilitySlideCropped.jpg Stability preliminary data]