Difference between revisions of "Part:BBa F2620"

Revision as of 18:26, 15 September 2006

3OC₆HSL -> PoPS Receiver

A transcription factor [LuxR] that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by an operator [TetR]. Device input is 3OC6HSL. Device output is PoPS produced at a LuxR-regulated operator.

Usage and Biology

Full PoPS output at high 3OC6HSL levels and high plasmid copy [e.g., Part:pSB1A2] results in a reduced cell growth rate (see Load section). If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Column 1	Column 2

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1004

Device Characteristics

control	R0040
device_type	Receiver
family	-NA-
latency	Seconds
proteins	LuxR, TetR
signalling_molecule	3OC₆HSL
switch_point	2nM

Get the device [http://openwetware.org/wiki/Image:F2620.de2-ania.ppt#filelinks datasheet]

Transfer Function

Latency

Specificity

[http://openwetware.org/images/9/93/Transfer.jpg Specificity preliminary data]

Stability

[http://openwetware.org/images/b/b9/StabilitySlideCropped.jpg Stability preliminary data]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_F2620 short</partinfo>
-A transcription factor [LuxR] that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC6HSL]] is controlled by an operator [TetR].  Device input is [[3OC6HSL|3OC6HSL]].  Device output is [[PoPS]] produced at a LuxR-regulated operator.
+A transcription factor [LuxR] that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC6HSL]] is controlled by an operator [TetR].  ''Device input'' is [[3OC6HSL|3OC6HSL]].  ''Device output'' is [[PoPS]] produced at a LuxR-regulated operator.
 ===Usage and Biology===