Difference between revisions of "Part:BBa K1433005:Experience"
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===Applications of BBa_K1433005=== | ===Applications of BBa_K1433005=== | ||
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| + | ===Validation of Chromosome Recombination with λ-Red Technique=== | ||
| + | <p>In order to test if the basic genome-editing part---λ-Red system works, we designed several different knock-in site pairs on E.coli chromosome and utilize antibiotic resistance gene to target them.</p> | ||
| + | <p>Firstly, from a large number of paper collection, we summarized aλ-Red protocol which tells that recombination bacteria cell preparation requires L-arabinose induction, and L-arabinose appears to inhibit cell growth due to cytotoxicity ofλ-Red proteins. Therefore, as to learn about cell growth circumstances under L-arabinose induction, and test ifλ-Red proteins get expressed on some level, we made a growth curve of gradient arabinose induced cell growth as follows. Through the curve, we can easily find that with the increase of arabinose induced concentration, cell grows slower and slower, implying the inhibition effect got strengthened.</p> | ||
| + | [[File:ZJU_ara induced growth curve.PNG|800px|thumb|center|'''Figure 1'''.The growth curves under L-arabinose gradients induction. Inhibition effect got strengthened with the increase of arabinose induced concentration.]] | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 19:42, 17 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1433005
Validation of Chromosome Recombination with λ-Red Technique
In order to test if the basic genome-editing part---λ-Red system works, we designed several different knock-in site pairs on E.coli chromosome and utilize antibiotic resistance gene to target them.
Firstly, from a large number of paper collection, we summarized aλ-Red protocol which tells that recombination bacteria cell preparation requires L-arabinose induction, and L-arabinose appears to inhibit cell growth due to cytotoxicity ofλ-Red proteins. Therefore, as to learn about cell growth circumstances under L-arabinose induction, and test ifλ-Red proteins get expressed on some level, we made a growth curve of gradient arabinose induced cell growth as follows. Through the curve, we can easily find that with the increase of arabinose induced concentration, cell grows slower and slower, implying the inhibition effect got strengthened.
User Reviews
UNIQ5c77174517f57c6f-partinfo-00000000-QINU UNIQ5c77174517f57c6f-partinfo-00000001-QINU