Difference between revisions of "Part:BBa K1554001"
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acyl-CoA + reduced acceptor + O2 = Delta11-acyl-CoA + acceptor + 2 H2O | acyl-CoA + reduced acceptor + O2 = Delta11-acyl-CoA + acceptor + 2 H2O | ||
| − | Our team used it in with [https://parts.igem.org/Part:BBa_K1554002 Part:BBa_K1554002] to produce the insect pheromone Z11-16:OH in Nicotiana benthamiana using palmitate:CoA as precursor. It was also used with [https://parts.igem.org/Part:BBa_K1554003 Part:BBa_K1554003] to transform Z11-16:OH into | + | Our team used it in with [https://parts.igem.org/Part:BBa_K1554002 Part:BBa_K1554002] to produce the insect pheromone Z11-16:OH in Nicotiana benthamiana using palmitate:CoA as precursor. It was also used with [https://parts.igem.org/Part:BBa_K1554003 Part:BBa_K1554003] to transform Z11-16:OH into another insect pheromone, Z11-16:OAc. |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 16:39, 7 October 2014
AtrΔ11
Delta 11 desaturase from Amyelois transitella. Codon optimization was perfomed for Nicotiana benthamiana.
Usage and Biology
The AtrΔ11 protein is a delta 11 desaturase coming from Amyelois transitella which introduces a desaturation in fatty acids at the 11th bond.
acyl-CoA + reduced acceptor + O2 = Delta11-acyl-CoA + acceptor + 2 H2O
Our team used it in with Part:BBa_K1554002 to produce the insect pheromone Z11-16:OH in Nicotiana benthamiana using palmitate:CoA as precursor. It was also used with Part:BBa_K1554003 to transform Z11-16:OH into another insect pheromone, Z11-16:OAc.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]