Difference between revisions of "Part:BBa K1554002"

Revision as of 16:38, 7 October 2014

HarFAR

HarFAR-3 fatty acid reductase from Helicoverpa armigera with an ER retention signal (KKYR) attached to the C-terminal end. Codon optimization was perfomed for Nicotiana benthamiana.

Usage and Biology

The HarFAR protein is a fatty acid reductase coming from Helicoverpa armigera which transforms fatty acids into fatty alcohols. Our team used it in with Part:BBa_K1554001 to produce the insect pheromone Z11-16:OH in Nicotiana benthamiana using palmitate:CoA as precursor. It was also used with Part:BBa_K1554003 to transform Z11-16:OH into another insect pheromone, Z11-16:OAc.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1104
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 929

@@ Line 7: / Line 7: @@
 The HarFAR protein is a fatty acid reductase coming from ''Helicoverpa armigera'' which transforms fatty acids into fatty alcohols.
-Our team used it in with [https://parts.igem.org/Part:BBa_K1554001 Part:BBa_K1554001] to produce the insect pheromone Z11-16:OH in ''Nicotiana benthamiana'' using palmitate:CoA as precursor. It was also used with [https://parts.igem.org/Part:BBa_K1554003 Part:BBa_K1554003] to transform Z11-16:OH into antoher insect pheromone, Z11-16:OAc.
+Our team used it in with [https://parts.igem.org/Part:BBa_K1554001 Part:BBa_K1554001] to produce the insect pheromone Z11-16:OH in ''Nicotiana benthamiana'' using palmitate:CoA as precursor. It was also used with [https://parts.igem.org/Part:BBa_K1554003 Part:BBa_K1554003] to transform Z11-16:OH into another insect pheromone, Z11-16:OAc.
 <span class='h3bb'>Sequence and Features</span>