Difference between revisions of "Part:BBa K1433001"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1433001 short</partinfo>
 
<partinfo>BBa_K1433001 short</partinfo>
  
 
Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1.  
 
Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1.  
This part is composed of 2 elements.  
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1. Promoter: BBa_J23110, a middle-ground Bacterial constitutive promoter.&#8232;
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This part is composed of 2 elements.:
 +
 
 +
1. Promoter: BBa_J23110, a middle-ground Bacterial constitutive promoter.
 +
 
 
2. attB and attP sites: Recognition site for Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase.
 
2. attB and attP sites: Recognition site for Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase.
This part promotes transcription and translation downstream gene, and when treat with Bxb1 gp35, upstream gene will express.
 
  
 +
This part promotes transcription and translation downstream gene, and when treat with Bxb1 gp35, upstream gene will express.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:05, 13 October 2014

attB-J23110-attP

Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1.

This part is composed of 2 elements.:

1. Promoter: BBa_J23110, a middle-ground Bacterial constitutive promoter.

2. attB and attP sites: Recognition site for Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase.

This part promotes transcription and translation downstream gene, and when treat with Bxb1 gp35, upstream gene will express.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 56
    Illegal NheI site found at 79
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 23
    Illegal BsaI.rc site found at 106