Difference between revisions of "Part:BBa K1486023"

(→‎Experiment 1: CpxR dimerization & Dimerization Orientation)
(→‎Associated Biobricks)
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<FONT SIZE="2"><P>: We assembled this part with the yeast resistance genes and the terminator ADH1. We also made a split sfGFP to evaluate the dimerization of hog1 and pbs2 in response to osmotic stress.
 
<FONT SIZE="2"><P>: We assembled this part with the yeast resistance genes and the terminator ADH1. We also made a split sfGFP to evaluate the dimerization of hog1 and pbs2 in response to osmotic stress.
 
<UL TYPE="CIRCLE">
 
<UL TYPE="CIRCLE">
−
<LI> sfGFP-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486026)
+
<LI> yeast-sfGFP-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486026)
−
<LI> sfGFP-N ( https://parts.igem.org/Part:BBa_K1486028)
+
<LI> yeast-sfGFP-N ( https://parts.igem.org/Part:BBa_K1486028)
−
<LI> sfGFP_N-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486029)
+
<LI> yeast-sfGFP_N-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486029)
−
<LI> sfGFP-ADH1-Ura ( https://parts.igem.org/Part:BBa_K1486032)
+
<LI> yeast-sfGFP-ADH1-Ura ( https://parts.igem.org/Part:BBa_K1486032)
−
<LI> sfGFP-C( https://parts.igem.org/Part:BBa_K1486034)
+
<LI> yeast-sfGFP-C( https://parts.igem.org/Part:BBa_K1486034)
−
<LI> sfGFP_C-ADH1-Ura( https://parts.igem.org/Part:BBa_K1486035)
+
<LI> yeast-sfGFP_C-ADH1-Ura( https://parts.igem.org/Part:BBa_K1486035)
 
</UL></P></FONT>
 
</UL></P></FONT>
  

Revision as of 10:14, 27 September 2014

Yeast optimized superfolder GFP

Purpose of the Biobrick

This part corresponds to the sequence of the superfolded GFP optimized for yeast cells. We used it as a tag to control the expression of our genes of interest Hog1 and Pbs2. It was extracted by PCR from plasmids we ordered to addgene:Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan and Plasmid 44873 pFA6a-link-yoSuperfolderGFP-CaURA3.


Experiment 1:

Associated Biobricks

: We assembled this part with the yeast resistance genes and the terminator ADH1. We also made a split sfGFP to evaluate the dimerization of hog1 and pbs2 in response to osmotic stress.

References

Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae. Lee et al (PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902. Print 2013. PubMed)



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2213
    Illegal PstI site found at 3446
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal PstI site found at 2213
    Illegal PstI site found at 3446
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3657
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1283
    Illegal XhoI site found at 2470
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2213
    Illegal PstI site found at 3446
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2213
    Illegal PstI site found at 3446
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1694
    Illegal AgeI site found at 2881
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961