Difference between revisions of "Part:BBa K1341001"

 
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__NOTOC__
 
<partinfo>BBa_K1341001 short</partinfo>
 
<partinfo>BBa_K1341001 short</partinfo>
 
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This system has proved that we have successful insert the BBa_K1341000 in the Afl III enzyme site(which has designed in the No.5 node sequence before this path way linked).Besides, this system also have a BamH I site in the No.2(start) point, a Nco I site in the No.8(terminal) point. The TetR device (BBa_K1341003) and LacI device (BBa_K1341002) can insert in this measurement system according to the requirement. By using the aTc and IPTG, we can prove whether the path way is the right result exactly such as this(2-4-5-7-8)
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CHA TU PIAN 1
 
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
===Usage and Biology===
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===The analysis about the logic of the system===
 
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CHA TU PIAN 2
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 04:51, 7 October 2014

PATH WAY TEST SYS IN BIO-COMPASS This system has proved that we have successful insert the BBa_K1341000 in the Afl III enzyme site(which has designed in the No.5 node sequence before this path way linked).Besides, this system also have a BamH I site in the No.2(start) point, a Nco I site in the No.8(terminal) point. The TetR device (BBa_K1341003) and LacI device (BBa_K1341002) can insert in this measurement system according to the requirement. By using the aTc and IPTG, we can prove whether the path way is the right result exactly such as this(2-4-5-7-8) CHA TU PIAN 1 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 11
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 180
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 837