Difference between revisions of "Part:BBa K1486024"

 
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<partinfo>BBa_K1486023 short</partinfo>
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===Purpose of the Biobrick===
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This part encodes for the KanMx resistance gene and confers dominant selection of geneticin to yeast. We fused it to our constructs to select the transformants after homologous recombinations. It was extracted by PCR from plasmids we ordered to addgene:Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan and Plasmid 44873 pFA6a-link-yoSuperfolderGFP-CaURA3.
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===Experiment 1:===
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===Associated Biobricks===
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<FONT SIZE="2"><P>This part was received with the terminator ADH1 and we added it to all our constructs being fused with the pbs2 gene.
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<UL TYPE="CIRCLE">
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<LI> yeast-sfGFP-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486026)
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<LI> RLuc-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486027)
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<LI> yeast-sfGFP_N-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486029)
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<LI> RLuc_N-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486030)
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===References===
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Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae. Lee et al (PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902. Print 2013. PubMed)
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K1486024 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1486008 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1486008 parameters</partinfo>
 
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Revision as of 16:18, 27 September 2014

Yeast optimized superfolder GFP

Purpose of the Biobrick

This part encodes for the KanMx resistance gene and confers dominant selection of geneticin to yeast. We fused it to our constructs to select the transformants after homologous recombinations. It was extracted by PCR from plasmids we ordered to addgene:Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan and Plasmid 44873 pFA6a-link-yoSuperfolderGFP-CaURA3.


Experiment 1:

Associated Biobricks

This part was received with the terminator ADH1 and we added it to all our constructs being fused with the pbs2 gene.

References

Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae. Lee et al (PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902. Print 2013. PubMed)



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2213
    Illegal PstI site found at 3446
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal PstI site found at 2213
    Illegal PstI site found at 3446
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3657
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1283
    Illegal XhoI site found at 2470
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2213
    Illegal PstI site found at 3446
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2213
    Illegal PstI site found at 3446
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1694
    Illegal AgeI site found at 2881
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961