Difference between revisions of "Part:BBa K1509003"
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The synthetic CP25 promoter was designed to be constitutive. CP25 is a stronge promoter in Lactococcus lactis by Jensen and Hammer.In the article,they measure the activities of the CP promoter in E. coli.The promoter activities were assayed from the expression of a reporter gene (lacLM) encoding β-galactosidase transcribed from the different synthetic promoter clones on the promoter cloning vector pAK80.The result suggests CP25 is the strongest promoter. | The synthetic CP25 promoter was designed to be constitutive. CP25 is a stronge promoter in Lactococcus lactis by Jensen and Hammer.In the article,they measure the activities of the CP promoter in E. coli.The promoter activities were assayed from the expression of a reporter gene (lacLM) encoding β-galactosidase transcribed from the different synthetic promoter clones on the promoter cloning vector pAK80.The result suggests CP25 is the strongest promoter. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | File:Nanocrystal.png | ||
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| + | fig1.CDS7 gene was under control of CP25 promoter. | ||
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| + | ===Result=== | ||
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| + | [[File:Nanocrystal2.png]] | ||
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| + | fig2.The ''E.coli'' in the feild of TEM. | ||
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Revision as of 01:55, 18 October 2014
CP25 is a highly active,constitutive promoter
The synthetic CP25 promoter was designed to be constitutive. CP25 is a stronge promoter in Lactococcus lactis by Jensen and Hammer.In the article,they measure the activities of the CP promoter in E. coli.The promoter activities were assayed from the expression of a reporter gene (lacLM) encoding β-galactosidase transcribed from the different synthetic promoter clones on the promoter cloning vector pAK80.The result suggests CP25 is the strongest promoter.
Usage and Biology
File:Nanocrystal.png
fig1.CDS7 gene was under control of CP25 promoter.
Result
fig2.The E.coli in the feild of TEM.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]