Difference between revisions of "Part:BBa K1445002:Design"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1445002 short</partinfo> | <partinfo>BBa_K1445002 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | Plasmid was designed to have the iGEM VF2 and VR sequencing sites along with translational and transcriptional terminators flanking the MCS. | + | Plasmid was designed to have the iGEM VF2 and VR sequencing sites along with translational and transcriptional terminators flanking the MCS. There are two amino acid changes from the published Litmus28i sequence located in the ampicillin resistance gene but these changes do not cause loss of function. |
Latest revision as of 03:10, 11 October 2014
High copy BioBricked M13 phagemid vector
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2990
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2996 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2990
Illegal BglII site found at 2852 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2990
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2990
Plasmid lacks a suffix.
Illegal XbaI site found at 3005
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1240
Design Notes
Plasmid was designed to have the iGEM VF2 and VR sequencing sites along with translational and transcriptional terminators flanking the MCS. There are two amino acid changes from the published Litmus28i sequence located in the ampicillin resistance gene but these changes do not cause loss of function.
Source
Limtus28i phagemid vector from NEB. Areas flanking MCS were cloned from pSB3C5 backbone.