Difference between revisions of "Part:BBa J100180:Experience"

Revision as of 13:18, 2 October 2014

In our experiment, we tested our P2(2) promoter against a positive control (lacI promoter), and a negative control (backwards promoter). Additionally, we tested each promoter at three pH levels (6.0, 7.0, 8.0) to see the effect of pH on the promoter.

The first graph shows successful transformation with the positive control (lacI) at all three pH levels with a significant drop in transformation in both the negative control and the experimental P2(2). The second graph compares our experimental P2(2) promoter against the negative control, and shows no significant difference between the two.

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 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+In our experiment, we tested our P2(2) promoter against a positive control (lacI promoter), and a negative control (backwards promoter).  Additionally, we tested each promoter at three pH levels (6.0, 7.0, 8.0) to see the effect of pH on the promoter.
-how you used this part and how it worked out.
-Here is our graph.
+The first graph shows successful transformation with the positive control (lacI) at all three pH levels with a significant drop in transformation in both the negative control and the experimental P2(2).
+The second graph compares our experimental P2(2) promoter against the negative control, and shows no significant difference between the two.
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