Difference between revisions of "Part:BBa K1365020:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Used primers: | + | Used primers: <br> |
− | forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTCAAAAGGAGAAGAGCT-3' | + | forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTCAAAAGGAGAAGAGCT-3' <br> |
− | reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTATAAAGCTCAT-3' | + | reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTATAAAGCTCAT-3' <br> |
− | to PCR sfGFP(Bs) from the plasmid and add the BioBrick prefix and suffix. | + | to PCR sfGFP(Bs) from the plasmid and add the BioBrick prefix and suffix. |
− | + | ||
− | + | ||
===Source=== | ===Source=== |
Revision as of 20:09, 24 September 2014
sfGFP(Bs)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 13
Design Notes
Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTCAAAAGGAGAAGAGCT-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTATAAAGCTCAT-3'
to PCR sfGFP(Bs) from the plasmid and add the BioBrick prefix and suffix.
Source
PCR on plasmid MG_sfgfp(Bs), used in the study 'Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging', see references.
Plasmid kindly provided by the Molecular Genetics group of the Rijksuniversiteit Groningen.