Difference between revisions of "Part:BBa K1365000:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
Used primers:
+
Used primers:<br>
−
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA-3'
+
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA-3'<br>
−
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA-3'
+
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA-3'<br>
−
to PCR NisA from the genome and add the BioBrick prefix and suffix.
+
to PCR NisA from the genome and add the BioBrick prefix and suffix.<br>
  
 
===Source===
 
===Source===

Revision as of 08:40, 23 September 2014


NisA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA-3'
to PCR NisA from the genome and add the BioBrick prefix and suffix.

Source

Obtained by PCR on the genome of L. lactis NZ9700, a nisin producing strain.

References