Difference between revisions of "Part:BBa K1491032"
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T7 Promoter with a RBS (BBa_S04423), RFP (BBa_E1010) and a T7 terminator (BBa_B0016). Allows RFP to be transcribed and translated when T7 RNA polymerase is present in the cell. | T7 Promoter with a RBS (BBa_S04423), RFP (BBa_E1010) and a T7 terminator (BBa_B0016). Allows RFP to be transcribed and translated when T7 RNA polymerase is present in the cell. |
Revision as of 16:22, 22 September 2014
T7 promoter-RBS-RFP-T7 Terminator
T7 Promoter with a RBS (BBa_S04423), RFP (BBa_E1010) and a T7 terminator (BBa_B0016). Allows RFP to be transcribed and translated when T7 RNA polymerase is present in the cell.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2525
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2950
Illegal AgeI site found at 2914 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2425