Difference between revisions of "Part:BBa K1399011"
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Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LVA-ssrA degradation tag and terminator.
 
Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LVA-ssrA degradation tag and terminator.
−
The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity.
+
The tagged RFP is actively degraded within cell (Figure 1), thus provides better temporal resolution of red fluorescence and promoter activity.
 
This part can be considered as improved version of [[Part:BBa_J04451]] as it does not contain StuI restriction site coding for additional 'RP' residues between RFP and LVA tag.
 
This part can be considered as improved version of [[Part:BBa_J04451]] as it does not contain StuI restriction site coding for additional 'RP' residues between RFP and LVA tag.
  
 +
[[File:EDiGEM14-SsrA degron mediated degradation.png|400px|thumb|center|'''Figure 1 SsrA degron mediated protein degradation.''' ('''A''') Any version of SsrA tags can be attached to any protein of interest (e.g. RFP or GFP). ('''B''') The tag is recognized by ClpX unfoldase forming a complex with ClpP protease and the tagged protein gets degraded.]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:30, 15 October 2014

Plac-RFP(LVA)

Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LVA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell (Figure 1), thus provides better temporal resolution of red fluorescence and promoter activity. This part can be considered as improved version of Part:BBa_J04451 as it does not contain StuI restriction site coding for additional 'RP' residues between RFP and LVA tag.

Figure 1 SsrA degron mediated protein degradation. (A) Any version of SsrA tags can be attached to any protein of interest (e.g. RFP or GFP). (B) The tag is recognized by ClpX unfoldase forming a complex with ClpP protease and the tagged protein gets degraded.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]