Difference between revisions of "Part:BBa K1398003"
Preadertor (Talk | contribs) |
|||
| Line 2: | Line 2: | ||
<partinfo>BBa_K1398003 short</partinfo> | <partinfo>BBa_K1398003 short</partinfo> | ||
| − | A construct created to | + | A construct created by the 2014 Exeter iGEM team to degrade TNT and nitroglycerin. |
The construct contains the coding sequence for NemA (BBa_K1398002), an enzyme involved in the degradation of toxic compounds for their reuse in nitrogen metabolism. | The construct contains the coding sequence for NemA (BBa_K1398002), an enzyme involved in the degradation of toxic compounds for their reuse in nitrogen metabolism. | ||
The construct also contains a Lactose-inducible promoter (BBa_R0010 ), a strong RBS (BBa_B0034) and a double terminator made up of BBa_B0010 and BBa_B0012. | The construct also contains a Lactose-inducible promoter (BBa_R0010 ), a strong RBS (BBa_B0034) and a double terminator made up of BBa_B0010 and BBa_B0012. | ||
The protein has been codon-optimised for expression in E. coli. | The protein has been codon-optimised for expression in E. coli. | ||
| + | |||
| + | The Exeter 2014 team used Raman spectroscopy to show the breakdown of Nitroglycerin by NemA: | ||
| + | |||
| + | https://static.igem.org/mediawiki/2014/6/67/NemA_degradation_of_nitroglycerin_kinetics_exp.png | ||
| + | |||
| + | The Exeter 2014 team also showed that NemA cannot perform this catalytic function without cofactors: FMN, NADH and NADPH. | ||
| + | |||
| + | https://static.igem.org/mediawiki/2014/e/ed/NG_Controls.jpg | ||
| + | |||
| + | Full article at: http://2014.igem.org/Team:Exeter/EnzymeValidation | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 12:41, 17 October 2014
NemA (Inducible Construct)
A construct created by the 2014 Exeter iGEM team to degrade TNT and nitroglycerin. The construct contains the coding sequence for NemA (BBa_K1398002), an enzyme involved in the degradation of toxic compounds for their reuse in nitrogen metabolism. The construct also contains a Lactose-inducible promoter (BBa_R0010 ), a strong RBS (BBa_B0034) and a double terminator made up of BBa_B0010 and BBa_B0012. The protein has been codon-optimised for expression in E. coli.
The Exeter 2014 team used Raman spectroscopy to show the breakdown of Nitroglycerin by NemA:
The Exeter 2014 team also showed that NemA cannot perform this catalytic function without cofactors: FMN, NADH and NADPH.
Full article at: http://2014.igem.org/Team:Exeter/EnzymeValidation
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 746
Illegal AgeI site found at 583 - 1000COMPATIBLE WITH RFC[1000]