Difference between revisions of "Part:BBa K1513000"
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| − | + | Our part is constituted by Plac、luxR、Pluxr、luxr. LuxI can result in the production of AHL—a kind of auto-inducer which can bind to the protein LuxR and induce the high expression of downstream genes of Pluxr，therefore，this system can drive a mass of bacteria highly express a particular gene. What’s more, AHL can be used by all bacteria in certain range. | |
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Revision as of 16:00, 24 September 2014
A quorum sensing system which can produce auto-inducer (AHL) itself.
Our part is constituted by Plac、luxR、Pluxr、luxr. LuxI can result in the production of AHL—a kind of auto-inducer which can bind to the protein LuxR and induce the high expression of downstream genes of Pluxr，therefore，this system can drive a mass of bacteria highly express a particular gene. What’s more, AHL can be used by all bacteria in certain range.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1710
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 987