Difference between revisions of "Part:BBa K1537027"

Revision as of 14:55, 17 September 2014

AdCP

Considering the problem of environment and safety, we use male sterility system which prevents the horizontal transgene flow. Pawan Shukla has used a plant pathogen-induced gene, cysteine protease to induce male sterility. This gene was identified in the wild peanut, Arachis diogoi differentially expressed when it was challenged with the late leaf spot pathogen, Phaeoisariopsis personata.

Arachis diogoi cysteine protease (AdCP) was expressed under the strong tapetum-specific promoter (TA29).And tobacco transformants were generated.
Morphological and histological analysis of AdCP transgenic plants showed ablated tapetum and complete pollen abortion.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 175

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1537027 short</partinfo>
-ATGGCTAGGCTCTCTTTATTGTTGTTACTTCTCGTGGCAGCTGTGGCTACCGCAGTTGATGATCAGGCAGATCCTCTCATTAGGCAGGTTACTGATGGAGATCATCACATGCTTAATGCTGAACATCACTTCACTACATTCAAGACTAAGTTCGGAAAGTCTTATGCTACACAAGAAGAGCATGATTACAGATTTGGTGTTTTCAGGGCAAACCTCAGAAGGGCTAAACTTCATGCAAAACTTGATCCTAGTGCTGAGCATGGAGTTACAAAGTTTTCTGATCTTACCCCAGAAGAGTTCAAGAGACAATATCTCGGTTTAAAACCTCTTAGGTTGCCATCAACCGCTAATAAGGCACCTATTCTCCCTACTTCTGATCTTCCTGAAAACTTTGATTGGAGAGATAAGGGAGCTGTTACTCCAGTGAAAAATCAAGGAAGTTGTGGTTCTTGCTGGGCTTTCAGTACCACTGGTGCTCTTGAAGGTGCACATTACTTGTCAACAGGTGAACTCGTTTCTTTATCAGAGCAACAGTTGGTTGATTGTGATCACGTGTGCGATCCTGAAGAGTATGGAGCTTGTGATGCAGGTTGCAACGGAGGTCTTATGAATAACGCTTTCGATTACATTTTGCAAGCAGGAGGTGTGCAGACCGAAAAGGATTATCCATACTCTGGAAGAGATGAGACATGTAAATTTGATAAGTCAAAGGTTGCTGCAACCGTGGCTAACTTCTCAGTTGTGAGTCTCGATGAAGATCAAATCGCTGCAAATCTCGTTAAGCATGGACCTTTAGCTGTGGGAATTAACGCAATTTTCATGCAGACATACATCGGAGGTGTTTCTTGTCCATACATATGCGGAAAAAACTTGGATCACGGTGTTCTTTTGGTGGGATATGGTGCTGCTGGATACGCTCCTATTAGATTCAAGGATAAGCCATTCTGGATTATCAAAAATTCTTGGGGAGAATCATGGGGAGAGGATGGTTATTATAAGATTTGTAGAGGAAAGAATGTTTGTGGTGTGGATTCAATGGTTTCATCTGTGGTTGCTACTACTTTCACTTCATCAAATAACTGA
+Considering the problem of environment and safety, we use male sterility system which prevents the horizontal transgene flow. Pawan Shukla has used a plant pathogen-induced gene, cysteine protease to induce male sterility. This gene was identified in the wild peanut, Arachis diogoi  differentially expressed when it was challenged with the late leaf spot pathogen, Phaeoisariopsis personata.
+ Arachis diogoi cysteine protease (AdCP) was expressed under the strong tapetum-specific promoter (TA29).And tobacco transformants were generated.
+ Morphological and histological analysis of AdCP transgenic plants showed ablated tapetum and complete pollen abortion.
 <!-- Add more about the biology of this part here