Difference between revisions of "Part:BBa K1529150"
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<partinfo>BBa_K1529150 parameters</partinfo> | <partinfo>BBa_K1529150 parameters</partinfo> | ||
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+ | ==Contribution From CAU_China 2022== | ||
+ | '''Group''': CAU_China,2022 https://2022.igem.org/Team:CAU_China | ||
+ | |||
+ | '''Author''': Liu Muhua | ||
+ | |||
+ | '''Summary''': Verified that this composite Part can produce oxalic acid in <i>Escherichia coli</i> | ||
+ | <br> | ||
+ | ===Characterization=== | ||
+ | <p>CAU_China 2022 used the CDS sequence in their oxalate secretion pathway and the expression product of <i>obcA</i> is one of the main enzyme in this pathway. It canact together with ObcB to catalyze oxaloacetic acid (OAA) and citric acid to form oxalic acid (OA). When there is only ObcA, it has a low ability to form oxalic acidin our project.</p> | ||
+ | <p>Hence, we aim to verify that the gene <i>obcA</i>, originally from <i> Burkholderia glumae</i>, can be regulated by PT7 and lac operator and properly expressed downstream to produce oxalic acid in <i>Escherichia coli</i>.</p> | ||
+ | <br> |
Revision as of 16:32, 27 September 2022
PT7_obcA
1
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 276
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 276
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1420
Illegal XhoI site found at 183
Illegal XhoI site found at 1307
Illegal XhoI site found at 1751 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 276
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 276
Illegal NgoMIV site found at 268
Illegal NgoMIV site found at 599
Illegal NgoMIV site found at 627
Illegal NgoMIV site found at 663
Illegal NgoMIV site found at 893
Illegal NgoMIV site found at 1670
Illegal NgoMIV site found at 1721
Illegal AgeI site found at 205 - 1000COMPATIBLE WITH RFC[1000]
Contribution From CAU_China 2022
Group: CAU_China,2022 https://2022.igem.org/Team:CAU_China
Author: Liu Muhua
Summary: Verified that this composite Part can produce oxalic acid in Escherichia coli
Characterization
CAU_China 2022 used the CDS sequence in their oxalate secretion pathway and the expression product of obcA is one of the main enzyme in this pathway. It canact together with ObcB to catalyze oxaloacetic acid (OAA) and citric acid to form oxalic acid (OA). When there is only ObcA, it has a low ability to form oxalic acidin our project.
Hence, we aim to verify that the gene obcA, originally from Burkholderia glumae, can be regulated by PT7 and lac operator and properly expressed downstream to produce oxalic acid in Escherichia coli.