Difference between revisions of "Part:BBa K1499500:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Since the luxI gene is under the expression of a lac-repressible promoter, in order to control expression of GFP, this construct must be used in lac deficient cells. | + | Since the luxI gene is under the expression of a lac-repressible promoter, in order to control expression of GFP, this construct must be used in lac deficient cells. |
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===Source=== | ===Source=== | ||
Latest revision as of 01:39, 15 September 2014
quorum sensing machinery that activates GFP expression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1815
Illegal BsaI.rc site found at 2543
Design Notes
Since the luxI gene is under the expression of a lac-repressible promoter, in order to control expression of GFP, this construct must be used in lac deficient cells.
Source
Combination of two previous parts: BBa_I13202 [1] and BBa_T9002 [2].