Difference between revisions of "Part:BBa K1499500"
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This part is the combination of two parts, BBa_I13202 and BBa_T9002. The first part (BBa_I13202) produces a luxI enzyme which produces an AHL (3OC6HSL). This part is under the control of a lac repressible promoter. The second part (BBa_T9002) produces luxR, which combines with the AHL to activate the luxPR promoter that controls expression of GFP.
 
This part is the combination of two parts, BBa_I13202 and BBa_T9002. The first part (BBa_I13202) produces a luxI enzyme which produces an AHL (3OC6HSL). This part is under the control of a lac repressible promoter. The second part (BBa_T9002) produces luxR, which combines with the AHL to activate the luxPR promoter that controls expression of GFP.
  
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http://2010.igem.org/File:Luxoperon.png
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[[File:luxoperon-cambridge2010.jpg]]
  
 
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Revision as of 20:35, 14 September 2014

quorum sensing machinery that activates GFP expression

This part is the combination of two parts, BBa_I13202 and BBa_T9002. The first part (BBa_I13202) produces a luxI enzyme which produces an AHL (3OC6HSL). This part is under the control of a lac repressible promoter. The second part (BBa_T9002) produces luxR, which combines with the AHL to activate the luxPR promoter that controls expression of GFP.

Luxoperon-cambridge2010.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1815
    Illegal BsaI.rc site found at 2543