Difference between revisions of "Part:BBa K1333204"

Revision as of 13:13, 24 September 2014

Or2-RBS-GFP-terminator

This is a reporter which combines plac Or2-62 and the generator of GFP. In the beginning of the Or2 sequence, there is a binding site for protein lambda cI. In Bacterial Two-hybrid System, the protein lambda cI is linked to a molecular called bait. And the corresponding target is linked to rpoA, which is the α subunit of RNA polymerase. The Or2 is reponsible for the recruitment of lambda cI-bait. When the bait interacts with the target, the rpoA is carried to downstream promoter and activates the downstream expression which is GFP in this case. Stronger interactions between baits and targets indicate higher concentration of rpoA near the promoter and higher level of transcription of GFP. Hence, we can tell the interactional degree between baits and targets from the fluorescence intensity of GFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 741

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1333204 short</partinfo>
-Fuse lambda cI with LGF2 mutated from Asp to Ala at site 78. As one of the components in Bacterial Two-hybrid System, it causes a less interaction with GAL11P compared to the nomalized WT.
+This is a reporter which combines plac Or2-62 and the generator of GFP. In the beginning of the Or2 sequence, there is a binding site for protein lambda cI. In Bacterial Two-hybrid System, the protein lambda cI is linked to a molecular called bait. And the corresponding target is linked to rpoA, which is the α subunit of RNA polymerase. The Or2 is reponsible for the recruitment of lambda cI-bait. When the bait interacts with the target, the rpoA is carried to downstream promoter and activates the downstream expression which is GFP in this case. Stronger interactions between baits and targets indicate higher concentration of rpoA near the promoter and higher level of transcription of GFP. Hence, we can tell the interactional degree between baits and targets from the fluorescence intensity of GFP.
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