Difference between revisions of "Part:BBa K1346004"
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Compared to the other alpha factor responsive promoters, pPRM1 has a low basal expression level, a broad range of expression, and the third highest maximum output, less than pAGA1 and pPCL2.
 
Compared to the other alpha factor responsive promoters, pPRM1 has a low basal expression level, a broad range of expression, and the third highest maximum output, less than pAGA1 and pPCL2.
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[[File:AFRP_GFP_DB_ROUND_4_140722.png|900px]]
 
[[File:AFRP_GFP_DB_ROUND_4_140722.png|900px]]

Revision as of 21:08, 3 September 2014

pPRM1

Promoter endogenous to s. cerevisiae that is induced by alpha factor. Characterized on flow cytometer by measuring its mean GFP fluorescence over 8 concentrations of alpha factor.

PRM1-zoomed.png

Compared to the other alpha factor responsive promoters, pPRM1 has a low basal expression level, a broad range of expression, and the third highest maximum output, less than pAGA1 and pPCL2.


AFRP GFP DB ROUND 4 140722.png

140722 AFRP GFP DB ROUND 4 zoomed.png

Alpha factor responsive promoters were selected from the following paper: Roberts, Christopher J., et al (2000 Feb 4). Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles. Science, 287(5454):873-80. http://www.ncbi.nlm.nih.gov/pubmed/10657304.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]