Difference between revisions of "Part:BBa K1346001"

Revision as of 20:42, 3 September 2014

pTEF1(m6) - constitutive promoter

Mutant of pTEF1 with differences in about 8 bp. High functioning compared to pTEF1 and its mutants m3, m7, & m10.

Data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to read GFP levels (arbitrary units)*

All mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae. Applied and Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 168

Revision as of 20:41, 3 September 2014 (view source) Jchsueh (Talk \| contribs) ← Older edit		Revision as of 20:42, 3 September 2014 (view source) Jchsueh (Talk \| contribs) Newer edit →
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	[[File:140630_pTEF1_GFP_ROUND_1.png\|900px]]		[[File:140630_pTEF1_GFP_ROUND_1.png\|900px]]

−	All mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae. Applied Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.	+	All mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in <i>Saccharomyces cerevisiae</i>. Applied and Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.