Difference between revisions of "Part:BBa K1484002:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
The AmajLime chromoprotein is now compatible with both standards and can be used more effectively.
 
  
 +
AmajLime  has been designed to be compatible with GoldenGate standards. This allows the part to be used in MoClo (ModularCloning) or GB2 (goldenbraid2) assembly method. These cloning techniques are based on based on Type IIS restriction enzymes that enable parallel assembly of multiple parts in a one-pot, one-step reaction and are becoming widely adopted across the field of Synthetic Biology.
  
 +
To be in the GoldenGate format, a level 0 CDS part must:
 +
start with
 +
-the BsaI_F recognition site
 +
  GGTCTC
 +
-one non-specific nucleotide
 +
  A
 +
-4bp fusion sequence (for CDS this is AATG)
 +
  AATG
 +
 +
finish with
 +
-4bp fusion sequence (for CDS this is    )
 +
  GCTT
 +
-one non-specific nucleotide
 +
  T
 +
-the BsaI_R recognition site reversed
 +
  GAGACC
 +
 +
 +
The AmajLime  GoldenGate biobrick reads:
 +
Prefix_GGTCTC A AATG ___tsPurple(w/o atg)___GCTT T GAGACC_Suffix
  
 
===Source===
 
===Source===

Latest revision as of 16:06, 15 October 2014


AmajLime chromoprotein in BBa and MoClo standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 706


Design Notes

AmajLime has been designed to be compatible with GoldenGate standards. This allows the part to be used in MoClo (ModularCloning) or GB2 (goldenbraid2) assembly method. These cloning techniques are based on based on Type IIS restriction enzymes that enable parallel assembly of multiple parts in a one-pot, one-step reaction and are becoming widely adopted across the field of Synthetic Biology.

To be in the GoldenGate format, a level 0 CDS part must: start with -the BsaI_F recognition site 

  GGTCTC

-one non-specific nucleotide 

  A

-4bp fusion sequence (for CDS this is AATG) 

  AATG

finish with -4bp fusion sequence (for CDS this is ) 

  GCTT

-one non-specific nucleotide 

  T

-the BsaI_R recognition site reversed 

  GAGACC


The AmajLime GoldenGate biobrick reads: Prefix_GGTCTC A AATG ___tsPurple(w/o atg)___GCTT T GAGACC_Suffix

Source

The chromoprotein was taken from the iGEM registry and altered to conform to the MoClo standard.

References