Difference between revisions of "Part:BBa K1346011"

Latest revision as of 21:26, 3 September 2014

pASG7

Promoter endogenous to s. cerevisiae that is induced by alpha factor. Characterized on flow cytometer by measuring its mean GFP fluorescence over 7 concentrations of alpha factor.

Compared to the other alpha factor responsive promoters, pASG7 has a high basal expression level, a sharply narrow range of expression, and a mid-level maximum output, comparable to pSAG1's maximum expression rate.

Alpha factor responsive promoters were selected from the following paper: Roberts, Christopher J., et al (2000 Feb 4). Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles. Science, 287(5454):873-80. http://www.ncbi.nlm.nih.gov/pubmed/10657304.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 252
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 252
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 252
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 252
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 [[File:ASG7-zoomed.png|900px]]
+Compared to the other alpha factor responsive promoters, pASG7 has a high basal expression level, a sharply narrow range of expression, and a mid-level maximum output, comparable to pSAG1's maximum expression rate.
 [[File:140722_AFRP_GFP_DB_ROUND_4_zoomed.png|900px]]
@@ Line 10: / Line 12: @@
-[[File:AFRP_GFP_DB_ROUND_4_140722.png|900px]]<!-- Add more about the biology of this part here
+[[File:AFRP_GFP_DB_ROUND_4_140722.png|900px]]
+Alpha factor responsive promoters were selected from the following paper: Roberts, Christopher J., et al (2000 Feb 4). Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles. Science, 287(5454):873-80. http://www.ncbi.nlm.nih.gov/pubmed/10657304.
+<!-- Add more about the biology of this part here
 ===Usage and Biology===