Difference between revisions of "Part:BBa K1442006"

 
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An aptazyme is a regulatory RNA element acting as a riboswitch in response to theophylline, a drug used to treat asthma and other chronic obstructive pulmonary diseases. Theophylline is not endogenous to the body therefore we used this element as a "kill switch" for our system.
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<p>An aptazyme is an allosteric ribozyme acting as a regulatory riboswitch. It self-splices in response to theophylline, a drug used to treat asthma and other chronic obstructive pulmonary diseases acting as a bronchodilator. This differs from caffeine by one methyl group and a nitrogen atom creating the structure seen below. This is not endogenous to the body therefore we used this element as a "kill switch" for our system.</p>
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One RNA molecule binds to theophylline with a dissociation constant of 0.1microM and
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 19:48, 11 October 2014

Anti-theophylline Aptazyme

An aptazyme is an allosteric ribozyme acting as a regulatory riboswitch. It self-splices in response to theophylline, a drug used to treat asthma and other chronic obstructive pulmonary diseases acting as a bronchodilator. This differs from caffeine by one methyl group and a nitrogen atom creating the structure seen below. This is not endogenous to the body therefore we used this element as a "kill switch" for our system.

One RNA molecule binds to theophylline with a dissociation constant of 0.1microM and Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]