Difference between revisions of "Part:BBa K1346002"
 
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<partinfo>BBa_K1346002 short</partinfo>
 
<partinfo>BBa_K1346002 short</partinfo>
  
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One of the mutant pTEF1 constitutive promoters characterized in http://aem.asm.org/content/72/8/5266.long. Seemingly the weakest out of the 5 that the UCSF/UCB team characterized.
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A mutant of the pTEF1 constitutive promoter. Seemingly the weakest out of the 5 that the UCSF/UCB team characterized.
  
 
[[File:140630_pTEF1_GFP_ROUND_1.png|900px]]
 
[[File:140630_pTEF1_GFP_ROUND_1.png|900px]]
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All mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in <i>Saccharomyces cerevisiae</i>. Applied and Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 20:43, 3 September 2014

pTEF1(m7) - constitutive promoter

A mutant of the pTEF1 constitutive promoter. Seemingly the weakest out of the 5 that the UCSF/UCB team characterized.

140630 pTEF1 GFP ROUND 1.png

All mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae. Applied and Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 169