Difference between revisions of "Part:BBa K1346003:Design"
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===References=== | ===References=== | ||
+ | Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae Elke Nevoigt1,2, Jessica Kohnke2, Curt R. Fischer1, Hal Alper1, Ulf Stahl2 and Gregory Stephanopoulos1,* | ||
+ | http://aem.asm.org/content/72/8/5266.long |
Latest revision as of 21:11, 5 August 2014
pTEF1(m10) - constitutive promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 169
Design Notes
Mutant of pTEF1
Source
Mutated from pTEF1 which is endogenous to the S. Cerevisiae genome. Mutants found in http://aem.asm.org/content/72/8/5266.long
References
Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae Elke Nevoigt1,2, Jessica Kohnke2, Curt R. Fischer1, Hal Alper1, Ulf Stahl2 and Gregory Stephanopoulos1,* http://aem.asm.org/content/72/8/5266.long