Difference between revisions of "Part:BBa K1346003"

 
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<partinfo>BBa_K1346003 short</partinfo>
 
<partinfo>BBa_K1346003 short</partinfo>
  
 
Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7. Characterization data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to gather data.
 
Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7. Characterization data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to gather data.
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[[File:140630_pTEF1_GFP_ROUND_1.png|900px]]
  
 
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Revision as of 21:17, 5 August 2014

pTEF1(m10) - constitutive promoter

Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7. Characterization data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to gather data.

140630 pTEF1 GFP ROUND 1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 169