Difference between revisions of "Part:BBa J176020:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
 
* Gel-shift characterization: The the DNA-binding stoichiometry of the 1-147 N-terminal portion of Gal4 is Gal4DB:UAS 17-mer = 2:1. [1]. The 17-mer is 5'-CGGAAGACTCTCCTCCG.
 
* Gel-shift characterization: The the DNA-binding stoichiometry of the 1-147 N-terminal portion of Gal4 is Gal4DB:UAS 17-mer = 2:1. [1]. The 17-mer is 5'-CGGAAGACTCTCCTCCG.
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* The Haynes lab UAS BioBrick [https://parts.igem.org/Part:BBa_J176019 5xGal4: BBa_J176019] has 5 copies of a similar 17-mer, each connected by 2 bp spacer: 5'-CGGAgtACTgTCCTCCG (ag)
+
* The Haynes lab UAS BioBrick [https://parts.igem.org/Part:BBa_J176019 5xGal4: BBa_J176019] has 5 copies of a similar 17-mer, each connected by 2 bp (spacer): 5'-CGGAgtACTgTCCTCCG (ag)
  
 
===Source===
 
===Source===

Latest revision as of 02:11, 22 July 2014

Gal4 DB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137


Design Notes

  • Gel-shift characterization: The the DNA-binding stoichiometry of the 1-147 N-terminal portion of Gal4 is Gal4DB:UAS 17-mer = 2:1. [1]. The 17-mer is 5'-CGGAAGACTCTCCTCCG.
  • The Haynes lab UAS BioBrick 5xGal4: BBa_J176019 has 5 copies of a similar 17-mer, each connected by 2 bp (spacer): 5'-CGGAgtACTgTCCTCCG (ag)

Source

TBA

References

1. Carey, M., Kakidani, H., Leatherwood, J., Mostashari, F., & Ptashne, M. (1989). An amino-terminal fragment of GAL4 binds DNA as a dimer. Journal of Molecular Biology, 209(3), 423–432.