Difference between revisions of "Part:BBa K1346000"

Line 4: Line 4:
 
Mutant of pTEF1 promoter with differences in about 26 bp. Fairly weak compared to pTEF1 and a few other mutants (m6,m10) , though stronger than M7.
 
Mutant of pTEF1 promoter with differences in about 26 bp. Fairly weak compared to pTEF1 and a few other mutants (m6,m10) , though stronger than M7.
  
[[File:140630 pTEF1 GFP ROUND 1.png]]
+
<img src="[[File:140630 pTEF1 GFP ROUND 1.png]]" height = "20"/>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 18:16, 15 July 2014

pTEF1(m3) - constitutive promoter

Mutant of pTEF1 promoter with differences in about 26 bp. Fairly weak compared to pTEF1 and a few other mutants (m6,m10) , though stronger than M7.

<img src="140630 pTEF1 GFP ROUND 1.png" height = "20"/> Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 169