Difference between revisions of "Part:BBa K1255003"

Revision as of 13:30, 17 June 2014

pConst + Riboswitch + BSMT1

BSMT1 opt. activated with a constitutive promotor, regulated a riboswitch.

BSMT1 (BBa_K1255001) encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase, which is a molecule catalyzes the salicylic acid to methyl salicylate. In order to do it, there must be added 10mM of salicylic acid to produce a winter green aroma. Besides this device has a constitutive promotor (BBa_J23119), it is regulated by a Riboswitch (BBa_K115017) that is a RBS but with a loop that unfolds at a temperature around of 32º C.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 474
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1255003 short</partinfo>
+BSMT1 opt. activated with a constitutive promotor, regulated a riboswitch.
 BSMT1 (BBa_K1255001) encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase, which is a molecule catalyzes the salicylic acid to methyl salicylate. In order to do it, there must be added 10mM of salicylic acid to produce a winter green aroma.  Besides this device has a constitutive promotor (BBa_J23119), it is regulated by a Riboswitch (BBa_K115017) that is a RBS but with a loop that unfolds at a temperature around of 32º C.