Difference between revisions of "Part:BBa K1256003"
 
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[[File:Mingdao_pSB1C3-Plac-SS-NB.png|400px]]
 
[[File:Mingdao_pSB1C3-Plac-SS-NB.png|400px]]
  
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The secretion signal (SS) of OmpA in E. coli were introduced on the vector of [https://parts.igem.org/Part:BBa_K1256001 BBa_K1256001]. The fusion protein with SS can be directed to secrete outside the engineered E. coli. NheI and BamHI sites can be used for cloning the coding sequence (cds). This vector is compatible with the standard BioBrick assembly rule, that is, parts can be exchanged using XbaI and PstI to the NheI and PstI on this vector.  
+
The secretion signal (SS) of OmpA in E. coli were introduced on the vector of [https://parts.igem.org/Part:BBa_K1256001 BBa_K1256001]. The fusion protein with SS can be produced and secreted outside the engineered E. coli to the culture media. NheI and BamHI sites can be used for cloning the coding sequence (cds). This vector is compatible with the standard BioBrick assembly rule, that is, parts can be exchanged using XbaI and PstI to the NheI and PstI on this vector.  
  
  

Latest revision as of 16:11, 9 June 2014

pSB1C3-Plac-SS-NB

Mingdao pSB1C3-Plac-SS-NB.png

The secretion signal (SS) of OmpA in E. coli were introduced on the vector of BBa_K1256001. The fusion protein with SS can be produced and secreted outside the engineered E. coli to the culture media. NheI and BamHI sites can be used for cloning the coding sequence (cds). This vector is compatible with the standard BioBrick assembly rule, that is, parts can be exchanged using XbaI and PstI to the NheI and PstI on this vector.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found at 1145
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3193
    Illegal NheI site found at 290
    Illegal SpeI site found at 1146
    Illegal PstI site found at 1160
    Illegal NotI site found at 1153
    Illegal NotI site found at 3199
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3193
    Illegal BamHI site found at 1002
    Illegal XhoI site found at 2177
    Illegal XhoI site found at 3069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3193
    Illegal suffix found at 1146
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3193
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3208
    Illegal SpeI site found at 1146
    Illegal PstI site found at 1160
    Illegal AgeI site found at 850
    Illegal AgeI site found at 962
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.