Difference between revisions of "Part:BBa K1256001"

Latest revision as of 15:17, 9 June 2014

pSB1C3-Plac-NB

NheI and BamHI sites were introduced just behind RBS (B0034) and in front of the terminator (B0015)on the vector of pSB1C3. RFP can easily be exchanged with other coding regions and maintain the standard BioBrick assembly rule (E-X-S-P). Furthermore, the front subpart (Plac-RBS) can be cut by EcoRI and NheI site and assembled with other BioBrick parts cut by EcoRI and XbaI. The back subpart (RFP-Tr) can be cut by NheI and PstI and assembled with other BioBrick parts cut by SpeI and PstI.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal suffix found at 1076
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3124
Illegal NheI site found at 221
Illegal SpeI site found at 1077
Illegal PstI site found at 1091
Illegal NotI site found at 1084
Illegal NotI site found at 3130
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3124
Illegal BamHI site found at 933
Illegal XhoI site found at 2108
Illegal XhoI site found at 3000
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 3124
Illegal suffix found at 1077
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 3124
Plasmid lacks a suffix.
Illegal XbaI site found at 3139
Illegal SpeI site found at 1077
Illegal PstI site found at 1091
Illegal AgeI site found at 781
Illegal AgeI site found at 893
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.

Revision as of 15:00, 9 June 2014 (view source) F91445122 (Talk \| contribs) ← Older edit		Latest revision as of 15:17, 9 June 2014 (view source) F91445122 (Talk \| contribs)
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	[[File:Mingdao PSB1C3-Plac-NB.png\|400px]]		[[File:Mingdao PSB1C3-Plac-NB.png\|400px]]

−	NheI and BamHI sites were introduced just behind RBS (B0034) and in front of the terminator (B0015)on the vector of pSB1C3. RFP can easily be exchanged with other coding ~~region~~ and maintain the standard BioBrick assembly rule (E-X-S-P). Furthermore, the front subpart (Plac-RBS) can be cut by EcoRI and NheI site and assembled with other BioBrick parts cut by EcoRI and XbaI. The back subpart (RFP-Tr) can be cut by NheI and PstI and assembled with other BioBrick parts cut by SpeI and PstI.	+	NheI and BamHI sites were introduced just behind RBS (B0034) and in front of the terminator (B0015)on the vector of pSB1C3. RFP can easily be exchanged with other coding regions and maintain the standard BioBrick assembly rule (E-X-S-P). Furthermore, the front subpart (Plac-RBS) can be cut by EcoRI and NheI site and assembled with other BioBrick parts cut by EcoRI and XbaI. The back subpart (RFP-Tr) can be cut by NheI and PstI and assembled with other BioBrick parts cut by SpeI and PstI.