Difference between revisions of "Part:BBa J97000"
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Revision as of 16:00, 6 February 2014
IP-Free EiraCFP (Cyan Fluorescent Protein)
This part was created by DNA 2.0 as part of their IP-Free series of fluorescent and chromogenic proteins. It is available to use under the BioBrick Public Agreement.
- Excitation 398nm, Emission 498nm
- Molecular Weight: 26.0kDA
- Length: 233 amino acids
EireCFP appears colorless to the naked eye, but has a light blue color under UV light.
EireCFP |
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Excitation and Emission Spectra |
Usage and Biology
DNA 2.0 created a poster to show how the parts were created which is available on their site here.DNA 2.0 IP-Free Fluorescent Protein Collection
Part BBa_J97000 is a member of a collection of four IP-Free fluorescent proteins. The four members of the collection have the following part numbers:
- EiraCFP: BBa_J97000
- JuniperGFP
- BlazeYFP
- TannenRFP
DNA2.0’s IP-Free© synthetic non-aequorea fluorescent proteins are intended to be used as a source of different fluorescent protein coding sequences (genes) that can be amplified by PCR and cloned into any other expression vector of choice.
Protein Collection Images under fluorescent light | Protein Collection Excitation and Emission Information |
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This part was contributed under the BioBrick Public Agreement (BPA). Click on the BioBrick Foundation logo to see more information about the BPA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
emission | 498 |
excitation | 398 |