Difference between revisions of "Part:BBa J119333"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J119333 short</partinfo> | <partinfo>BBa_J119333 short</partinfo> | ||
| − | The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (M6C from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. | + | The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (M6C from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. Mutation of A to G at position 152 nt in RBS element. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 15:20, 5 February 2014
Ammeline Biosensor with Riboswitch M6C (A172G)
The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (M6C from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. Mutation of A to G at position 152 nt in RBS element.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 740
Illegal AgeI site found at 852 - 1000COMPATIBLE WITH RFC[1000]