Difference between revisions of "Part:BBa J23105"

Revision as of 22:06, 3 October 2006

constitutive promoter family member

Variant RFP (au) J23112 1 J23103 17 J23107 908 J23113 21 J23106 1185 J23109 106 J23108 1303 J23117 162 J23118 1429 J23114 256 J23111 1487 J23115 387 J23101 1791 J23116 396 J23104 1831 J23105 623 J23102 2179 J23110 844 J23100 2547

Constitutive promoter family
Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. J23119 is the "consensus" promoter sequence and the strongest member of the family. All parts except J23119 are present in plasmid J61002. Part J23119 is present in pSB1A2. This places the RFP downstream of the promoter. Reported activities of the promoters are given as the relative fluorescense of these plasmids in strain TG1 grown in LB media to saturation. See part J61002 for details on their use.

These promoter parts can be used to tune the expression level of constitutively expressed parts. The NheI and AvrII restriction sites present within these promoter parts make them a scaffold for furhter modification.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_J23105 short</partinfo>
-Later
+[[Image:BerkiGEM2006-Promoters.jpg|300px|left]]
+[[Image:BerkiGEM2006-PromotersEppendorfs.jpg|300px]]<br>
+<tt>
+Variant RFP (au)
+J23112 1   <br>
+J23103 17  J23107 908<br>
+J23113 21  J23106 1185<br>
+J23109 106 J23108 1303<br>
+J23117 162 J23118 1429<br>
+J23114 256 J23111 1487<br>
+J23115 387 J23101 1791<br>
+J23116 396 J23104 1831<br>
+J23105 623 J23102 2179<br>
+J23110 844 J23100 2547<br>
+<br>
+</tt>
+----
+[[Image:pBca1020-r0040.jpg|left|180px]]<br>
+'''Constitutive promoter family'''<br>
+Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library.  J23119 is the "consensus" promoter sequence and the strongest member of the family.  All parts except J23119 are present in plasmid J61002.  Part J23119 is present in pSB1A2.  This places the RFP downstream of the promoter.  Reported activities of the promoters are given as the relative fluorescense of these plasmids in strain TG1 grown in LB media to saturation.  See part J61002 for details on their use.
+These promoter parts can be used to tune the expression level of constitutively expressed parts.  The NheI and AvrII restriction sites present within these promoter parts make them a scaffold for furhter modification.
 <!-- Add more about the biology of this part here