Difference between revisions of "Part:BBa K1021007"
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https://static.igem.org/mediawiki/2013/6/60/Cochliobolus_fluorescence_composite.png
 
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===Characterization===
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<b>DTU-Denmark 2019 characterization</b>
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Revision as of 19:40, 21 October 2019

PtrpC

PtrpC is a strong constitutive promoter from Aspergillus nidulans's tryptophan biosynthesis pathways. It was isolated using a fungal transformation vector and constructed into the pSB1C3 backbone for continuous homologous and heterologous gene expression in fungal chassis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


To test the activity of the trpC promoter, a composite part was created with GFP downstream (BBa_K1021023). This part was transformed into the ascomycete fungi Cochliobolus heterostrophus. Results indicated that the trpC promoter provided GFP expression within this fungal chassis.

Cochliobolus_fluorescence_composite.png


Characterization

DTU-Denmark 2019 characterization