Difference between revisions of "Part:BBa K1119010:Design"

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===Source===
 
===Source===
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The EF-1alpha promoter sequence was cloned out from plasmid iDUET101a (http://www.addgene.org/browse/sequence_vdb/2491/) using PCR with primers that includes prefix and suffix in RFC10 standard.
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The EF-1alpha promoter sequence was cloned out from plasmid iDUET101a (http://www.addgene.org/17629/) using PCR with primers that includes prefix and suffix in RFC10 standard.
  
 
===References===
 
===References===
 
Qin, Jane Yuxia, Li Zhang, et al. "Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter." PLoS ONE. 5.5 (2010) <http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010611>.
 
Qin, Jane Yuxia, Li Zhang, et al. "Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter." PLoS ONE. 5.5 (2010) <http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010611>.

Revision as of 03:21, 29 October 2013


Human Elongation Factor-1alpha Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 83
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Not applicable

Source

The EF-1alpha promoter sequence was cloned out from plasmid iDUET101a (http://www.addgene.org/17629/) using PCR with primers that includes prefix and suffix in RFC10 standard.

References

Qin, Jane Yuxia, Li Zhang, et al. "Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter." PLoS ONE. 5.5 (2010) <http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010611>.