Difference between revisions of "Part:BBa K1129046"
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<h2>Background</h2>
 
<h2>Background</h2>
  
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<p> 4-coumarate 3-hydroxylase is an enzyme in the vanillin pathway. 4-coumarate 3-hydroxylase (4-CMH) hydrolyses p-coumaric acid to caffeic acid. It is the 2nd enyzme the biosynthetic pathway of vanillin from the amino acid tyrosine.</p>  
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<p> 4-coumarate 3-hydroxylase is an enzyme in the vanillin pathway. 4-coumarate 3-hydroxylase (4-CMH) hydrolyses p-coumaric acid to caffeic acid. It is the 2nd enzyme the biosynthetic pathway of vanillin from the amino acid tyrosine. 4-CMH is biobrick (<html><b><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K238007">BBa_K238007</b></a></html>) from <html><b><a href="http://2009.igem.org/Team:KULeuven/Design/Vanillin_Production">KULeuven iGEM 2009</b></a></html> </p>  
  
  
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Figure 3. Compound generation identification by GC-MS. Chromatograms and mass spectra for select peaks are shown. Structures represent predictions based on library matching or comparison to standards. Controls represent plasmids missing the gene of interest. A) Internal control using caffeic acid. B) Conversion of p-coumaric acid to caffeic acid by constitutive expressed 4CMH. C) Possible conversion of p-coumaric acid to caffeic acid by inducibly expressed 4CMH. The mass spectrum however is confounded by another compound.
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Figure 3. Compound generation identification by GC-MS. Chromatograms and mass spectra for select peaks are shown. Structures represent predictions based on library matching or comparison to standards. Controls represent plasmids missing the gene of interest. A) Internal control using caffeic acid. B) Conversion of p-coumaric acid to caffeic acid by constitutive expressed 4-CMH. C) Possible conversion of p-coumaric acid to caffeic acid by inducibly expressed 4CMH. The mass spectrum however is confounded by another compound.

Revision as of 01:50, 29 October 2013

4-coumarate 3-hydroxylase under pTET constitutive promoter


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 100
    Illegal NgoMIV site found at 108
    Illegal NgoMIV site found at 858
    Illegal NgoMIV site found at 1031
    Illegal NgoMIV site found at 1433
    Illegal NgoMIV site found at 1437
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 632


Background

4-coumarate 3-hydroxylase is an enzyme in the vanillin pathway. 4-coumarate 3-hydroxylase (4-CMH) hydrolyses p-coumaric acid to caffeic acid. It is the 2nd enzyme the biosynthetic pathway of vanillin from the amino acid tyrosine. 4-CMH is biobrick (BBa_K238007) from KULeuven iGEM 2009



Experimental Data



PhpGF9BoJPM%281%29.jpg


Figure 3. Compound generation identification by GC-MS. Chromatograms and mass spectra for select peaks are shown. Structures represent predictions based on library matching or comparison to standards. Controls represent plasmids missing the gene of interest. A) Internal control using caffeic acid. B) Conversion of p-coumaric acid to caffeic acid by constitutive expressed 4-CMH. C) Possible conversion of p-coumaric acid to caffeic acid by inducibly expressed 4CMH. The mass spectrum however is confounded by another compound.