Difference between revisions of "Part:BBa K737024:Experience"
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We improved this OUC-China’s phosphate sensor part including the <i>phoB</i> promoter, by amplifying the <i>phoA</i> promoter region of <i>E. coli</i> (MG1655) and ligating it upstream of GFP part (<partinfo>BBa_K1139200</partinfo>)<br> | We improved this OUC-China’s phosphate sensor part including the <i>phoB</i> promoter, by amplifying the <i>phoA</i> promoter region of <i>E. coli</i> (MG1655) and ligating it upstream of GFP part (<partinfo>BBa_K1139200</partinfo>)<br> | ||
− | [[Image: | + | [[Image:Titech2013_part_K1139201_Fig4.jpg|thumb|left|240px|'''Fig. 1.''' Our <i>phoA</i> promoter assay result]] |
− | [[Image: | + | [[Image:Titech2013_part_K1139201_Fig5.jpg|thumb|left|240px|'''Fig. 2.''' OUC-China 2012’s <i>phoB</i> promoter assay result (converted to bar chart)]] |
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Compared to OUC-China’s phosphate sensor part including the <i>phoB</i> promoter (Fig. 2), our phosphate sensor part shows a clearer result (Fig. 1) (<partinfo>BBa_K1139200</partinfo>, <partinfo>BBa_K1139201</partinfo>). | Compared to OUC-China’s phosphate sensor part including the <i>phoB</i> promoter (Fig. 2), our phosphate sensor part shows a clearer result (Fig. 1) (<partinfo>BBa_K1139200</partinfo>, <partinfo>BBa_K1139201</partinfo>). |
Revision as of 16:19, 28 October 2013
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UNIQ480a192974ce1bdc-partinfo-00000000-QINU UNIQ480a192974ce1bdc-partinfo-00000001-QINU
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Tokyo-Tech iGEM 2013 |
1. Sequence 2. Result of our experiment
Compared to OUC-China’s phosphate sensor part including the phoB promoter (Fig. 2), our phosphate sensor part shows a clearer result (Fig. 1) (BBa_K1139200, BBa_K1139201). |