Difference between revisions of "Part:BBa J31007:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| − | This sequence was amplified from | + | This sequence was amplified from pSB1AT3 and is identical to the Tet resistance gene found in pBR322. BBa_J31007 was cloned into the pSB1A2 plamsid. |
===Source=== | ===Source=== | ||
Revision as of 06:38, 30 October 2006
tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 144
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 290
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 316
Illegal NgoMIV site found at 684
Illegal NgoMIV site found at 844 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence was amplified from pSB1AT3 and is identical to the Tet resistance gene found in pBR322. BBa_J31007 was cloned into the pSB1A2 plamsid.
Source
pSB1AT3