Difference between revisions of "Part:BBa K1088000"

Revision as of 11:28, 28 October 2013

1-deoxyxylulose-5-phosphate synthase (Dxs) enzyme from MEP pathway isolated from B. subtilis 168

The MEP pathway is a common bacterial isoprenoid pathway. To improve the yield of the end product, isopentenyl diphosphate (IPP) and dimethylallyl pyrophosphate(DMAPP), Zhao L et al. expressed, in 2011, the 1-deoxyxylulose-5-phosphate synthase(Dxs) gene as well as the enzyme catalyzing the second step of the MEP pathway. They observed a 2.3 fold increase in isoprene output (isoprene is one of many products from the MEP pathway).

Source: Lishan Zhao, Wei-chen Chang, Youli Xiao, Hung-wen Liu and Pinghua Liu: Methylerythritol Phosphate Pathway of Isoprenoid Biosynthesis, Annu. Rev. Biochem. 2013. 82:497-530, page 500. [http://www.ncbi.nlm.nih.gov/pubmed/23746261 (link)]

From Biocyc: "Dxs catalyzes the thiamin diphosphate-dependent reaction that condenses pyruvate and D-glyceraldehyde-3-phosphate to yield 1-deoxy-D-xylulose 5-phosphate [Julsing07]. This is the first, rate-limiting step in the methylerythritol phosphate pathway of isoprenoid biosynthesis, and is believed to feed into the pyridoxal 5-phosphate (vitamin B6) and thiamin (vitamin B1) biosynthesis pathways." http://biocyc.org/BSUB/NEW-IMAGE?type=ENZYME&object=BSU24270-MONOMER Source.]

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1063
Illegal EcoRI site found at 1720
Illegal PstI site found at 1121
Illegal PstI site found at 1567
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1063
Illegal EcoRI site found at 1720
Illegal PstI site found at 1121
Illegal PstI site found at 1567
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1063
Illegal EcoRI site found at 1720
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1063
Illegal EcoRI site found at 1720
Illegal PstI site found at 1121
Illegal PstI site found at 1567
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1063
Illegal EcoRI site found at 1720
Illegal PstI site found at 1121
Illegal PstI site found at 1567
Illegal NgoMIV site found at 1020
Illegal AgeI site found at 913
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 862
Illegal SapI.rc site found at 1561

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1088000 short</partinfo>
-The MEP pathway is a common bacterial isoprenoid pathway. To improve the yield of the end product, isopentenyl diphosphate (IPP) and dimethylallyl pyrophosphate(DMAPP), Zhao et al. expressed, in 2011, the 1-deoxyxylulose-5-phosphate synthase(Dxs) gene as well as the enzyme catalyzing the second step of the MEP pathway. They observed a 2.3 fold increase in isoprene output (isoprene is one of many products from the MEP pathway).
+The MEP pathway is a common bacterial isoprenoid pathway. To improve the yield of the end product, isopentenyl diphosphate (IPP) and dimethylallyl pyrophosphate(DMAPP), Zhao L et al. expressed, in 2011, the 1-deoxyxylulose-5-phosphate synthase(Dxs) gene as well as the enzyme catalyzing the second step of the MEP pathway. They observed a 2.3 fold increase in isoprene output (isoprene is one of many products from the MEP pathway).
+Source: Lishan Zhao, Wei-chen Chang, Youli Xiao, Hung-wen Liu and Pinghua Liu: Methylerythritol Phosphate Pathway of Isoprenoid Biosynthesis, Annu. Rev. Biochem. 2013. 82:497-530, page 500.
+[http://www.ncbi.nlm.nih.gov/pubmed/23746261 (link)]
 From Biocyc:
 "Dxs catalyzes the thiamin diphosphate-dependent reaction that condenses pyruvate and D-glyceraldehyde-3-phosphate to yield 1-deoxy-D-xylulose 5-phosphate [Julsing07]. This is the first, rate-limiting step in the methylerythritol phosphate pathway of isoprenoid biosynthesis, and is believed to feed into the pyridoxal 5-phosphate (vitamin B6) and thiamin (vitamin B1) biosynthesis pathways."
--http://biocyc.org/BSUB/NEW-IMAGE?type=ENZYME&object=BSU24270-MONOMER
+http://biocyc.org/BSUB/NEW-IMAGE?type=ENZYME&object=BSU24270-MONOMER Source.]