Difference between revisions of "Part:BBa K1129001"
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<partinfo>BBa_K1129001 parameters</partinfo> | <partinfo>BBa_K1129001 parameters</partinfo> | ||
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| + | <h2>Background</h2> | ||
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| + | Ferulic acid is best utilized as a precursor for synthesis of aromatic compounds such as vanillin, which involves 2 enzymes: the conversion of ferulic acid to feruloyl-CoA by feruloyl-CoA synthetase (Fcs) enzymes and the conversion of feruloyl-CoA into vanillin by enoyl-CoA hydratase/aldolase (Ech) enzymes. BBa_K1129001 is a feruloyl-CoA-synthetase (fcs) from ''Pseudomonas putida KT2440'' which converts ferrulic acid to feruloyl CoA. | ||
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| + | <html><center><IMG id="emp" SRC="https://static.igem.org/mediawiki/2013/b/bf/UBC-Vanillin-structure.png" USEMAP="#vin"> | ||
| + | <map id="vin" name="vin"><area shape="rect" alt="" title="" coords="104,101,242,184" href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1129000" target="" /><area shape="rect" alt="" title="" coords="353,103,491,189" href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1129046" target="" /><area shape="poly" alt="" title="" coords="595,76,750,77,750,150,696,151,699,263,647,263,644,164,595,164" href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1129041" target="" /><area shape="rect" alt="" title="" coords="489,387,630,487" href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1129024" target="" /><area shape="rect" alt="" title="" coords="205,384,351,487" href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1129022" target="" /><!-- Created by Online Image Map Editor (http://www.maschek.hu/imagemap/index) --></map> | ||
| + | </center></html> | ||
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| + | <h2>Experimental Data</h2> | ||
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| + | <p align=center>https://static.igem.org/mediawiki/2013/a/ac/Ech900.jpg</p> | ||
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| + | '''Figure 1''' Compound generation identification by GC-MS. Chromatograms(left) and mass spectra(Right) for select peaks are shown. Structures represent predictions based on library matching or comparison to standards. Controls represent plasmids missing the gene of interest. A) Internal control using vanillin(10.486). B) Conversion of Ferulic acid to vanillin acid by constitutive expressed Fcs and Ech through the Feuroyl CoA intermediate, not shown. C) Conversion of Ferulic acid to vanillin acid by Arabinose inducible expressed Fcs and Ech through the Feuroyl CoA intermediate, not shown. | ||
Revision as of 03:03, 25 October 2013
Feruloyl-CoA-Synthetase (FCS) from Pseudomonas putida KT2440
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 43
Illegal XhoI site found at 1153 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 30
Illegal NgoMIV site found at 294
Illegal NgoMIV site found at 772
Illegal NgoMIV site found at 1027
Illegal NgoMIV site found at 1090
Illegal NgoMIV site found at 1107 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 802
Background
Ferulic acid is best utilized as a precursor for synthesis of aromatic compounds such as vanillin, which involves 2 enzymes: the conversion of ferulic acid to feruloyl-CoA by feruloyl-CoA synthetase (Fcs) enzymes and the conversion of feruloyl-CoA into vanillin by enoyl-CoA hydratase/aldolase (Ech) enzymes. BBa_K1129001 is a feruloyl-CoA-synthetase (fcs) from Pseudomonas putida KT2440 which converts ferrulic acid to feruloyl CoA.
Experimental Data
Figure 1 Compound generation identification by GC-MS. Chromatograms(left) and mass spectra(Right) for select peaks are shown. Structures represent predictions based on library matching or comparison to standards. Controls represent plasmids missing the gene of interest. A) Internal control using vanillin(10.486). B) Conversion of Ferulic acid to vanillin acid by constitutive expressed Fcs and Ech through the Feuroyl CoA intermediate, not shown. C) Conversion of Ferulic acid to vanillin acid by Arabinose inducible expressed Fcs and Ech through the Feuroyl CoA intermediate, not shown.