Difference between revisions of "Part:BBa K1159313"
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===Usage and Biology===
 
===Usage and Biology===
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The so called N-terminal coded 6xHis-Tag consists of six histidine residues and allows the C-terminal fusion proteins for protein purification after protein expression in E. coli.  
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The so called N-terminal encoded 6xHis-Tag consists of six histidine residues and allows the C-terminal fusion proteins for protein purification after protein expression in E. coli. The iGEM Team of TU Munich 2013 used the 6x His-Tag to purify several recombinant proteins, which have been expressed in E. coli, by affinity chromatographie.  
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The iGEM Team of TU Munich 2013 used the 6x His-Tag to purify several recombinant proteins, which have been expressed in E. coli, by affinity chromatographie.  
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Protocol for affinity purification of 6xHis-tagged proteins:
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 15:09, 23 October 2013

N-terminal His6-tag in RFC[25] N-Part

This parts codes for a N-terminal His6-tag flanked by RFC[25] N-part to allow C-terminal fusions


Usage and Biology

The so called N-terminal encoded 6xHis-Tag consists of six histidine residues and allows the C-terminal fusion proteins for protein purification after protein expression in E. coli. The iGEM Team of TU Munich 2013 used the 6x His-Tag to purify several recombinant proteins, which have been expressed in E. coli, by affinity chromatographie.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]