Difference between revisions of "Part:BBa K1159313"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | The so called N-terminal coded 6xHis-Tag consists of six histidine residues and allows the C-terminal fusion proteins for protein purification after protein expression in E. coli. | |
| + | The iGEM Team of TU Munich 2013 used the 6x His-Tag to purify several recombinant proteins, which have been expressed in E. coli, by affinity chromatographie. | ||
| + | Protocol for affinity purification of 6xHis-tagged proteins: | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 14:45, 23 October 2013
N-terminal His6-tag in RFC[25] N-Part
This parts codes for a N-terminal His6-tag flanked by RFC[25] N-part to allow C-terminal fusions
Usage and Biology
The so called N-terminal coded 6xHis-Tag consists of six histidine residues and allows the C-terminal fusion proteins for protein purification after protein expression in E. coli. The iGEM Team of TU Munich 2013 used the 6x His-Tag to purify several recombinant proteins, which have been expressed in E. coli, by affinity chromatographie.
Protocol for affinity purification of 6xHis-tagged proteins:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]