Difference between revisions of "Part:BBa K1031922"

 
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__NOTOC__
 
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<partinfo>BBa_K1031922 short</partinfo>
 
<partinfo>BBa_K1031922 short</partinfo>
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<p>For detailed information concerning XylB, please visit <a href="http://2013.igem.org/Team:Peking/Project/Plugins">2013 Peking iGEM Adaptors</a></p>
  
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J23114-XylB-Terminator
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<img src="https://static.igem.org/mediawiki/igem.org/c/c9/Peking_Logo.jpg" style="width:960px;"/>
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== '''Characterization''' ==
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We achieved the concept of Adaptor by connecting XylC with XylS, and we want to further confirm whether other degradation enzymes could be added. So we construct XylB adaptor circuit adopting <html><a href="https://parts.igem.org/Part:BBa_J23114">J23114</a></html> as constitutive promoter and <html><a href="https://parts.igem.org/Part:BBa_B0015">B0015</a></html> as terminator ('''Fig 1''')
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<img src="https://static.igem.org/mediawiki/2013/0/01/Peking2013_part_114-XylB.png" style="width:450px; margin-left:220px" />
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<p style="text-align:center"><b>Fig 1</b> Construction of adaptor circuit <i>Pc</i>/J23109-XylB. The orange arrowhead refers to constitutive <i>Pc</i> promoter. RBS is shown in green oval. The square in dark red stands for terminator B0015.
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</html>
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== '''Sequence and Features''' ==
  
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1031922 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1031923 SequenceAndFeatures</partinfo>
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== '''Data shown''' ==
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('''Fig 2''').
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<img src="" style="width:600; margin-left:150px" />
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<p style="text-align:center"><b>Fig 2</b>
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1031922 parameters</partinfo>
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<partinfo>BBa_K1031621 parameters</partinfo>
 
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Revision as of 16:19, 22 October 2013

J23114-XylB-Terminator

For detailed information concerning XylB, please visit 2013 Peking iGEM Adaptors


Characterization

We achieved the concept of Adaptor by connecting XylC with XylS, and we want to further confirm whether other degradation enzymes could be added. So we construct XylB adaptor circuit adopting J23114 as constitutive promoter and B0015 as terminator (Fig 1)

Fig 1 Construction of adaptor circuit Pc/J23109-XylB. The orange arrowhead refers to constitutive Pc promoter. RBS is shown in green oval. The square in dark red stands for terminator B0015.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 364


Data shown

(Fig 2).

Fig 2