Difference between revisions of "Part:BBa K1104203:Experience"

Revision as of 13:46, 21 October 2013

Introduction

This part: AhpCp1000 is one of the improvement versions of BioBrick Part: ahpC promoter (Part:K362001) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCp1000 (Part:BBa_K1104203) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema ceranae].

AhpCp1000 +E0840 (Part:BBa_K1104243)

The reporter: AhpCp1000 +E0840 (Part:BBa_K1104243) includes promoter (Part:BBa_K1104203) and Part:BBa_E0840. We induce the promoter by different concentrations of H₂O₂ to identify the strength. After AhpCp1000 (Part:BBa_K1104203) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.

We tested the improved promoter: AhpCp1000 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. This wiki page shows the protocols we used for promoter testing.

Results

This is the relative promoter strength of BBa_K1104203 under different H202 concentrations tested using BBa_K11042043 (BBa_K1104203 +E0840) and found that there is a trend where the higher the H₂0₂ concentration, the higher the promoter expression:

Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101:

Comparison between ahpC and its improvements. (Part:BBa_K1104243)

Promoter testing of ahpC promoter (Part:K362001) and its improvements.

Applications of BBa_K1104203

User Reviews

UNIQ6aa6fb1ae3877fa1-partinfo-00000000-QINU UNIQ6aa6fb1ae3877fa1-partinfo-00000001-QINU

@@ Line 7: / Line 7: @@
 [[File: NYMU_43.png|thumb|400px|center|'''AhpCp1000 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104243 Part:BBa_K1104243])''']]
-We use the device: AhpCp1000 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104243 Part:BBa_K1104243]) includes promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104203 Part:BBa_K1104203]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840], induce the promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and identify the strength. After AhpCp1000 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104203 Part:BBa_K1104203]) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.
+The reporter: AhpCp1000 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104243 Part:BBa_K1104243]) includes promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104203 Part:BBa_K1104203]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840]. We induce the promoter by different concentrations of H<sub>2</sub>O<sub>2</sub> to identify the strength. After AhpCp1000 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104203 Part:BBa_K1104203]) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.
 We tested the improved promoter: AhpCp1000 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. This wiki page shows the protocols we used for promoter testing.